Evaluation of Different Amino Acids on Growth and Cyanide Production by Bacillus megaterium for Gold Recovery

Bio-cyanidation, as a sustainable and effective method to extract gold from primary secondary resources, has attracted attention because of its environmental friendliness economic benefits. The effect amino acids on bio-cyanide production using Bacillus megaterium (B. megaterium) is less explored area in this field the main interest study. Here, glycine, threonine, glutamine over concentration range 0 10 g/L was investigated. results showed at equal (5 g/L), glycine yields (maximum ca. 110 mg/L) higher biogenic cyanide (bio-CN), while threonine produce 74 mg/L 64 mg/L, respectively). For first time, optimization mixing three investigated revealed more significant roles for stimulation bio-CN by B. megaterium. interactions involved biosynthesis were explained with reference metabolic pathways cycle bacteria. In mixed acids, optimum medium identified be 2.84 3.0 absence which could high 86 bio-CN, resulting leaching efficiency comparable chemical cyanide.